Treatment of spinach Photosystem I particles with detergents induced an apparent blue shift of chlorophyll α at 690 nm in the difference spectrum of P-700. As a consequence of the band shift, the differential absorption coefficient of P-700 was increased from 63 to 87 mM-1 .cm-1. A curve was presented, with which changes in the apparent differential absorption coefficient of P-700 can be estimated by measuring magnitudes of light-induced absorption decreases at 680 nm and 700 nm. The curve was compared with that for cyanobacterial preparations and the mechanism of the detergent-induced band shift was discussed.
|ジャーナル||Plant and Cell Physiology|
|出版ステータス||Published - 1990|
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