Water-soluble conjugate of double-stranded DNA and poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins

Daisuke Umeno; Masafumi Kawasaki; Mizuo Maeda

doi:10.1021/bc980019f

Water-soluble conjugate of double-stranded DNA and poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins

Daisuke Umeno, Masafumi Kawasaki, Mizuo Maeda^*

^*この研究の対応する著者

研究成果: Article › 査読

50 被引用数 (Scopus)

抄録

Poly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 °C. This system was used for the one-pot separation of restriction endonuclease EcoRI.

本文言語	English
ページ（範囲）	719-724
ページ数	6
ジャーナル	Bioconjugate Chemistry
巻	9
号	6
DOI	https://doi.org/10.1021/bc980019f
出版ステータス	Published - 1998
外部発表	はい

ASJC Scopus subject areas

バイオテクノロジー
バイオエンジニアリング
生体医工学
薬理学
薬科学
有機化学

文献へのアクセス

10.1021/bc980019f

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引用スタイル

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