@inproceedings{5c4c176b7ab14814ad7c4ac6849dbbd6,
title = "Yeast-based ligand assay system for detecting G protein-coupled receptor activation in water-in-oil droplets",
abstract = "We present a yeast-based ligand assay system to detect activation of G protein-coupled receptors (GPCRs) in water-in-oil (W/O) droplets. A microfluidic device is used to encapsulate template DNA encoding peptides, a cell-free coupled transcription-translation system, and budding yeast cells expressing GPCR into W/O droplets. The yeast cells are genetically engineered to express green fluorescent protein (GFP) via their intracellular signaling pathway in response to ligand stimulation of GPCR. We successfully demonstrated that the synthesized peptide ligand activated the cognate GPCR in W/O droplets. This will be a new platform for identifying novel peptide agonists for mammalian GPCRs.",
keywords = "Cell-free protein synthesis, Droplet, GPCR, Ligand assay, Yeast",
author = "Takashi Sakurai and Ryo Iizuka and Yasuyuki Tanigaki and Rui Sekine and Yoon, {Dong Hyun} and Tetsushi Sekiguchi and Jun Ishii and Akihiko Kondo and Naoto Nemoto and Shuichi Shoji and Takashi Funatsu",
year = "2012",
month = jan,
day = "1",
language = "English",
isbn = "9780979806452",
series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
publisher = "Chemical and Biological Microsystems Society",
pages = "1537--1539",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",
}